Glucose estimation

Bertrand s reagents (glucose estimation) a) 40 g of copper sulfate diluted to 1 liter (fc) rochelle salt 200 g, NaOH 150 g, and sufficient water to make 1 liter (c) ferric sulfate 50 g, H2SO4 200 g, and sufficient water to make 1 liter d) KMn04 5 g and sufficient water to make 1 liter. 

Bang s reagent (for glucose estimation) dissolve 100 g of K2C03, 66 g of KC1, and 160 of KHCOj in the order given in about 700 mL of water at 30°C. Add 4.4 g of copper sulfate and dilute to 1 liter after the C02 is evolved. This solution should be shaken only in such a manner as not to allow the entry of air. After 24 hours 300 mL diluted to a liter with saturated KC1 solution, shaken gently and used after 24 hours 50 mL = 10 mg glucose. 

Fig. 28.1. Reproduced from Fig. 5 of Ref. [43], with permission from Elsevier. The relationship between ultimate pH and glycogen concentration in muscle from 51 bulls. The values labelled glucose were derived from glucose estimates returned by the ESPRIT glucometer. Those labelled glucose-plus-lac-tate include estimates of the lactate levels in the tissue. In practice, decisions about glycogen and pH are based only on glucose estimates.

Medicine is becoming increasingly an objective and quantitative science, and like other sciences demands a realistic appraisal of the value of the numerical data upon which its conclusions are based. No longer is it permissible to regard a result of a blood glucose estimation, for instance, as an absolute value beyond question. Instead, such a figure must... 

Intermediate Monitor. Glycosylated (ketoamine) hemoglobin assays provide an overview of control for the previous 5 to 8 weeks. Such assays are independent of patient compliance an assay every 2 to 3 months will also reduce the number of blood samplings required for blood glucose estimation. 

Besides electrochemical methods, the hydrogen peroxide can also be measured with optical methods. Recently, glucose estimation was done according the Saifer-Gerstenfield method using optical readout and the enzyme peroxidase ... 

Bertrand s reagent (glucose estimation). Consists of the following solutions ... 

Starch may be detected by the blue coloration it gives with iodine solution, and confirmed by microscopical exanaination, or it may be converted into glucose by inversion, and the glucose estimated by means of Pehling s solution. 

Benzidine hydrochloride solution (for sulfate determination). Prepare a paste of 8 g of benzidine hydrochloride (Cj2Hg(NHj)2 2HCl) and 20 mL of distilled water, add 20 mL of 20% (mass/mass) HCl and dilute to 1 L with distilled water. Each mL of this solution is equivalent to 0.00357 g of H SO. Note that the reagent is often called benzidine dihydrochloride. Bertrand s reagent (glucose estimation). Consists of the following solutions ... 

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