Global conformational changes

The CP MAS NMR spectroscopy has been also extensively used for studies of proteins containing retinylidene chromophore like proteorhodopsin or bacteriorhodopsin. Bacteriorhodopsin is a protein component of purple membrane of Halobacterium salinarium.71 7 This protein contains 248 amino acids residues, forming a 7-helix bundle and a retinal chromophore covalently bound to Lys-216 via a Schiff base linkage. It is a light-driven proton pump that translocates protons from the inside to the outside of the cell. After photoisomerization of retinal, the reaction cycle is described by several intermediate states (J, K, L, M, N, O). Between L and M intermediate states, a proton transfer takes place from the protonated Schiff base to the anionic Asp85 at the central part of the protein. In the M and/or N intermediate states, the global conformational changes of the protein backbone take place. 

Using Analytical Ultracentrifugation (AUC) to Measure Global Conformational Changes Accompanying Equilibrium Tertiary Folding of RNA Molecules... 

Global Conformational Changes of SSI Induced by Complex Formation with Subtilisin... 

Gallagher, S. C., Callaghan, A. J., Zhao, J., Dalton, H., and Trewhella, J., 1999, Global Conformational Changes Control the Reactivity of Methane Monooxygenase, Biochemistry 38 6572fi6760. 

E. Eisenstein, D.W. Markby, and H.K. Schachman. 1990. Heterotropic effectors promote a global conformational change in aspartate transcarbamoylase Biochemistry 29 3724-3731. (PubMed)... 

W.E. Werner and H.K. Schachman. 1989. Analysis of the ligand-promoted global conformational change in aspartate transcarbamoylase Evidence for a two-state transition from boundary spreading in sedimentation velocity experiments J. Mol. Biol. 206 221-230. (PubMedl... 

Lsciistem. E-, Markby, D. W.. and Schachman, H. K. 1490. Hcterolropic effectors promote a global conformational change in aspartate transcarbamoylase. Biochemistry 29 3724—3731. 

Polymerases Undergo a Global Conformational Change upon dNTP Binding 352... 

Photoisomerization of the retinal from the ] -trans to the l3-cis form initiates proton transfer steps from the cytoplasmic to the extracellular side via the J, K, L, M, N, and O intermediates of a cyclic reaction.These intermediate states can be distinguished over picosecond to millisecond ranges, and a series of the cycle is completed in less than 10 ms. Global conformational changes of the protein... 

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