Global amplification methods

Here, we describe the details of an improved cDNA subtraction method based on a cDNA subtraction approach previously applied to single cells (6), which in turn is based on a global cDNA amplification protocol (PolyAPCR) used to produce amplified cDNAs, representing all the mRNAs present in the samples as small as a single cell (7,8). PolyAPCR has been applied successfully to a wide range of samples, including single micromanipulated cells (6,7,9,10), antibody fractionated populations (11), and fixed tissues (12). 

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