GlcNAc intersecting

Compound 14 may be isolated from the urine of patients with Sand-hoff s disease (GM2-gangliosidosis variant O).62 Fundamentally, it is a diantennary oligosaccharide bearing an additional, so-called intersecting GlcNAc residue (9), /3-(l—>4)-l inked to Man-3. The 500-MHz, H-n.m.r. spectrum of 14 is given in Fig. 15, and the n.m.r. data are presented in Table VII. 

H Chemical Shifts of Structural-reporter Groups of Constituent Monosaccharides for Two Oligosaccharides (14 and 15) and a Glycopeptide (16) Containing the Intersecting GlcNAc Residue (9)... 

Compound 15 is another diantennary oligosaccharide, having the intersecting GlcNAc-9 linked /3-(l—>4) to Man-3. It has been isolated from the urine of a patient with64,65 Morquio syndrome type B. The 500-MHz, xH-n.m.r. spectrum of 15 is presented in Fig. 16, and the chemical shifts of the structural-reporter groups are included in Table VII. 

Fig. 2. Structures of glycans found in Dictyostelium discoideum. Structures of known carbohydrate components in Dictyostelium discoideum. The structures have been determined by physical/chemical methods or by the analysis of radiolabeled materials. (A) Lipid-linked oligosaccharide precursor of N-linked oligosaccharides [14] (B) truncated lipid-linked oligosaccharide precursor found in mutant strains HL24I and HL243[I4] (C) oligosaccharide with GlcNAc residues at the intersecting position and locations of the methylphosphate esters [16].

---