Gibbs free energy, protein stability

Section 6.1 considered the noncovalent binding energies that stabilize a protein strnctnre. However, the folding of a protein depends ultimately on the difference in Gibbs free energy (AG) between the folded (F) and unfolded (U) states at some temperature T ... 

In the following, we elucidate what thermodynamic properties are required for calculating the stability diagram of a protein. The Gibbs free energy difference between the unfolded and native state is defined as ... 

Protein stability has generally been defined in terms of the free energy change between the native and the unfolded states (AGu). This parameter of unfolding can be used to decipher stability of the native state across a wide temperature range.43 The relation that incorporates all of the terms described above is the modified Gibbs-Helmholtz equation and is a function of temperature. 

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