Genotypically Unstable Mutants

An unexpected finding was that petite induction in strain 1121 was not 

It was of interest to determine the effects of the petite-inducing conditions described above on mitochondrial inner-membrane proteins, and, in particular, on products of mitochondrial protein synthesis. The efficient and quantitative production of petites at 18°C affords a special opportunity to examine the fate of mitochondrial translation products during the course 

Double-label CsCl whole-cell DNA gradients of strains 183 and 184. Cells grown on 2% galactose were incubated for 15 min with 200 /ig/ml of cycloheximide and labeled for 2.5 h with [ H]adenine (183, A------A) or P C]adenine (184, ------- ). Spheroplasts were pre- 

The necessity of mitochondrial translation products for stability of the mitochondrial genome can be visualized either as a direct requirement—for 

There is now considerable evidence indicating a close interdependence between cytoplasmic- and mitoribosomal-directed proteins for the assembly and function of inner-membrane complexes like cytochrome oxidase and the oligomycin-sensitive ATPase. Thus an alteration in a nuclear gene product could profoundly affect the level or functioning of a mitochondrial translation product or products such a situation could apply as well to the replication of wild-type mtDNA. 

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