General principles signal intensity

The principle of this pulse method and its general equations are easily extended to the case of several components in a mixture. The method was used by Lindholm et al. [24] to determine the quaternary isotherms of the enantiomers of methyl- and ethyl-mandelate on the chiral phase Chiral AGP. One of the serious roadblocks encountered in the use of the pulse tracer method is that the amplitudes of most of the system peaks decrease rapidly when the plateau concentration increases. Since the signal noise increases in the same time, it becomes rapidly impossible to make any accurate measurements of the retention time of these peaks. On the basis of fundamental work by Tondeur et al. [114], the origin of this variation of the relative intensity of the system peaks was explained by Forss n et al. [47], who then derived an effective rule to determine the composition of a perturbation pulse that generates system peaks that are detected easily. The concentrations of the components in the injected perturbation pulse should... 

Time-domain techniques record the intensity of the signal as a function of time, frequency-domain techniques record the phase and the amplitude of the signal as a function of frequency. Time domain and frequency domain are connected via the Fourier transform. Therefore, the time domain and the frequency domain are generally equivalent. However, this does not imply an equivalence between time-domain and frequency-domain recording techniques or the instruments used for each. An exhaustive comparison of the techniques is difficult and needs to include a number of different electronic design principles and applications. 

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