General principles of pulse and phase-modulation fluorometries

General principles of pulse and phase-modulation fluorometries 

Pulse fluorometry The sample is excited by a short pulse of light and the fluorescence response is recorded as a function of time. If the duration of the pulse is long 

8) The convolution integral appearing in this equation can be easily understood by considering the excitation function as successive Dirac functions at various times t. 

Phase-modulation fluorometry The sample is excited by a sinusoidally modulated light at high frequency. The fluorescence response, which is the convolution product (Eq. 6.9) of the S-pulse response by the sinusoidal excitation function, is sinusoidally 

Relationship between harmonic response and rt-pulse response It is worth demonstrating that the harmonic response is the Fourier transform of the d-pulse response. The sinusoidal excitation function can be written as 

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