Interphase chromosomes gene localization

Table 1 lists representative examples of the application of in situ hybridization, including FISH, to studies that use chromosome preparations and interphase nuclei (4,5). FISH is superior to in situ hybridization with an enzymatic probe because it provides finer resolution and higher signal intensity. This is especially important when the localization of a gene within a specific chromosome band is to be established. 

Fluorescence In Situ Hybridization is a technique that utilizes fluorescently modified DNA probes to hybridize to specific sequences in metaphase chromosome preparations or interphase nuclei. These probes can be used to localize single-gene copies, teleomeres, centromeres, and specific whole chromosomes... 

---