Gene Expression mRNA Quantification and Transcriptome Analysis

2 Gene expression mRNA quantification and transcriptome analysis 

An improvement in DD-PCR can be made by the use of restriction enzyme digestion of double-stranded cDNA followed by ligation of an adapter to mediate selective PCR 

A candidate mixture of nucleic acids of differing sequences is prepared. The candidate molecules consist of 5 - and 3 -regions of fixed sequences and the middle regions of randomized sequences. The fixed sequences are chosen either  

5 Constan Random sequence y Constant Library of random DNA sequence  

The candidate mixture is contacted with a selected target under the condition favorable for binding between the target and the members of the candidate mixture. 

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