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Fura-2 loaded HEL cells

Figure 3 Characterization of the inhibitory effect of HRLRY-NH2 (compound 32) on the NPY-induced increase in cytosolic Ca2+ in fura-2 loaded HEL cells. (A) Effect of NPY (100 nM) on the increase in cytosolic Ca2+ in HEL cells. (Reproduced from Mol. Pharmacol. 1992 41, 767-771.) (B) Comparative potencies of NPY ( ), the Y1-receptor-specific agonist [Leu31,Pro34]NPY (O) and the Y2-receptor agonist NPY13-36 ( ) on the increase in cytosolic Ca2+ in HEL cells. (Reproduced from Mol. Pharmacol. 1992 41, 767-771.) (C)C1 cytosolic Ca2t increase in response to a half-maximal concentration (5 nM) of NPY. C2 cytosolic Ca2+ response of 5 nM NPY 30 s after addition of 1 pM HRLRY-NH2 to the medium. Cl and C2 represent separate cuvettes. (Reproduced from Mol. Pharmacol. 1995 48, 425-432.) (D) Dose-response curves for the NPY-induced increase in cytosolic Ca2+ in the presence ( ) and absence (O) of 1.0 pM HRLRY-NH2. The inhibitor was added to the medium 30 s prior to stimulation with NPY (5 nM). (Reproduced from Mol. Pharmacol. 1995 48,425-432.) Results in panels C and D are presented as the 340/380 nm fluorescence ratio, and represent a typical experiment repeated with different cell preparations and with similar results (for detailed experimental procedure, see Daniels dal., 1992). Figure 3 Characterization of the inhibitory effect of HRLRY-NH2 (compound 32) on the NPY-induced increase in cytosolic Ca2+ in fura-2 loaded HEL cells. (A) Effect of NPY (100 nM) on the increase in cytosolic Ca2+ in HEL cells. (Reproduced from Mol. Pharmacol. 1992 41, 767-771.) (B) Comparative potencies of NPY ( ), the Y1-receptor-specific agonist [Leu31,Pro34]NPY (O) and the Y2-receptor agonist NPY13-36 ( ) on the increase in cytosolic Ca2+ in HEL cells. (Reproduced from Mol. Pharmacol. 1992 41, 767-771.) (C)C1 cytosolic Ca2t increase in response to a half-maximal concentration (5 nM) of NPY. C2 cytosolic Ca2+ response of 5 nM NPY 30 s after addition of 1 pM HRLRY-NH2 to the medium. Cl and C2 represent separate cuvettes. (Reproduced from Mol. Pharmacol. 1995 48, 425-432.) (D) Dose-response curves for the NPY-induced increase in cytosolic Ca2+ in the presence ( ) and absence (O) of 1.0 pM HRLRY-NH2. The inhibitor was added to the medium 30 s prior to stimulation with NPY (5 nM). (Reproduced from Mol. Pharmacol. 1995 48,425-432.) Results in panels C and D are presented as the 340/380 nm fluorescence ratio, and represent a typical experiment repeated with different cell preparations and with similar results (for detailed experimental procedure, see Daniels dal., 1992).
Figure 4 Dose-response curves for the NPY ( ED50=3.3 nM) and YINLIYRLRY-NH2 (compound 58) ( ED50=8.7 nM) induced increase in cytosolic Ca2+ in fura-2 loaded HEL cells. Results are presented as the 340/380 nm fluorescence ratio. Figure 4 Dose-response curves for the NPY ( ED50=3.3 nM) and YINLIYRLRY-NH2 (compound 58) ( ED50=8.7 nM) induced increase in cytosolic Ca2+ in fura-2 loaded HEL cells. Results are presented as the 340/380 nm fluorescence ratio.
Figure 10 Inhibition of the NPY-induced increase in cytosolic calcium in fura-2 loaded HEL cells by GW 1229 (compound 84). (Left) C1 cytosolic calcium response to a half-maximal dose ofNPY (5 nM). C2 cytosolic calcium response to NPY 30 s after the addition of 0.5 nM compound 84. Cl and C2 represent separate cuvettes. (Right) NPY dose-response curves in the presence (H) and absence (Q) of 84 at 0.25 nM. (Reproduced from Proc. Mali Acad. Sci. USA 1995 92, 9067-9071.)... Figure 10 Inhibition of the NPY-induced increase in cytosolic calcium in fura-2 loaded HEL cells by GW 1229 (compound 84). (Left) C1 cytosolic calcium response to a half-maximal dose ofNPY (5 nM). C2 cytosolic calcium response to NPY 30 s after the addition of 0.5 nM compound 84. Cl and C2 represent separate cuvettes. (Right) NPY dose-response curves in the presence (H) and absence (Q) of 84 at 0.25 nM. (Reproduced from Proc. Mali Acad. Sci. USA 1995 92, 9067-9071.)...
See also in sourсe #XX -- [ Pg.134 , Pg.137 , Pg.140 , Pg.146 ]



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