Fungal endopolygalacturonases

Analysis of the interaction between PGIP from Phaseolus vulgaris L and fungal endopolygalacturonases using biosensor technology... 

Pectic substances derived from the cell wall by the action of fungal endopolygalacturonase have been identified as el-icitors (Croteau and Johnson, 1985). 

Figure 1. Transverse section of barley leaf epidermal cells taken perpendicular to the long axis of the cells and anticlinal to the leaf surface. The section has been labeled by the EMSIL technique (see Methods) utilizing purified C. sativus endopolygalacturonase and monoclonal antibody EPG-4, which is specific for this enzyme, in order to localize the substrate of the enzyme at the typical site penetrated by the fungal pathogen. Bar = 1 pm. Inset Comparable cell wall region as in Fig. 1 but labeled with monoclonal antibody JIM 5 to localize non-esterified pectin. Bar = 1 pm. Note the identical labeling patterns obtained with either method.

Chemical constituents present include sterols, mainly ergosterol (0.3-0.4%) and er-gosterol peroxide, 3-sitosterol, 24-methylcho-lesta-7,22-dien-3- 3-ol, and other sterol esters, fungal lysozyme, acid protease, and other enzymes (laccase, endopolygalacturonase, cellulase, amylase, etc.) water-soluble protein, polypeptides amino acids trehalose and other sugars mannitol betaine adenosine alkanes (tetracosane, hentriacontane) and fatty acids (tetracosanoic, stearic, palmitic, nonadecanoic, and behenic acids) (jIANGSU wang). ... 

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