Functional bioassays

The best CB2-selective agonists to have been developed to date are all non-eicosanoid cannabinoids (Howlett et al. 2002 Ibrahim et al. 2003 Pertwee 1999a). They include the classical cannabinoids, L-759633, L-759656 and JWH-133, the non-classical cannabinoid HU-308, and the aminoalkylindole AM1241 (Figs. 5,6 and 7). All these ligands bind more readily to CB2 than to CBi receptors (Table 2) and have also been shown to behave as potent CB2-selective agonists in functional bioassays (Hanus et al. 1999 Ibrahim et al. 2003 Pertwee 2000 Ross et al. 1999a). 

Schwartzbauer JE (1991) Alternative splicing of fibronectin three variants, three functions. Bioassay 13 527-533... 

Goodnow The general message I get from putting Jean-Laurent Casanova s paper together with yours is that when you find these missense amino acid substitutions, unless you have a good functional bioassay it will be tough to interpret it much. 

Most of the work at Molecular Devices Corporation on the microflow chamber/LAPS combination has used living cells as the biological component. The rate R(t) of pH change in the chamber is a measure of the metabolic rate of cells, which is coupled directly or indirectly to almost all events that affect the cells [19]. The result is a functional bioassay with applications in pharmacology [32], characterizing receptor/ligand interaction [33],... 

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