Fucosylation with Integrated Cofactor Regeneration

To overcome the synthetic and inhibition problems, an integrated recycling system is applied. GTP and Fuc-l-P are transformed by GDP-fucosyl-pyro-phosphorylase to pyrophosphate and the activated donor (GDP-Fuc) [71]. This in turn in the presence of al-3/4-fucosyltransferase fucosylates the acceptor Gal)3l-4GlcNAc at the GlcNAc-3-position or the acceptor Gal)3l-3GlcNAc at the GlcNAc-4-position. The released GDP is phosphorylated to GTP with pyruvate kinase employing the activated phosphate donor phosphenol pyruvate, which in 

An associated enzyme, al-3-fucosyltransferase, has also been used in syntheses to provide the Lewis x and sialyl Lewis x structural motifs [65]. Increasing availability of fucosyltransferases via cloning techniques are expected to have a strong influence on oligosaccharide synthesis in the near future. 

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Fig. 8. Enzymatic fucosylation with integrated cofactor regeneration (Enzymes vi cf. Fig. 2, xi GDP-fucose-pyrophosphorylase, xii al-3/4-fucosyltransferase) [72]... 

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