Freezing solutes and

Immerse the chuck with the tissue slice into the freezing solution. Freezing is indicated by a change in color of the embedding solution from clear to white and will be completed in 5-10 s. After 10 s, remove the chuck and tissue from the freezing solution, and place them on dry ice (see Note 5). 

The principles of this method were first published by White. " The mixture of solution and solid solvent is contained under near adiabatic conditions. Thermal equilibrium between the phases is maintained by a method of stirring which is efficient but causes the minimum of heating. When equilibrium is achieved the temperature is recorded and a solid-free sample of solution is withdrawn for analysis. In the differential method, a temperature difference is measured between the freezing solution and pure freezing solvent, measured under identical conditions of heat loss and gain. 

Resuspend cell pellet in 1 mL of freezing solution, and dispense 0.5 mL of suspension into two 1-mL cryotubes. 

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See also in sourсe #XX -- [ , ]

See also in sourсe #XX -- [ , ]

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