Fragment marking

Comparison of the reaction transform (a) and fragment-marking (b) approaches to the enumeration of izoles. 

Enumeration is the computational equivalent of carrying out a combinatorial synthesis. The result is a virtual library of product molecules that can then be analyzed using a library design program to select compounds of interest. Two different approaches to library enumeration have been developed fragment marking and the reaction-transform approach (14). 

TUNEL stands for terminal deoxynucleotidyl transferase x-dUTP nick end labeling. This assay is based on the detection of DNA fragments marked by an enzyme that incorporates modified nucleotides to the 3 -OH ends of the fragments, which can be then specifically detected. The enzyme is a deoxynucleotidyl transferase, which can act in absence of a complementary strand. Among the nucleotides, there is one specifically marked with a fluorochrome, an enzyme, or an antigen. This allows different methods of detection. 

FIGURE I The 5 -terminal sequences of pNOSS l and pNOSS 2 are encoded by closely linked exons separated from a common exon 2 by a large intron. The human genomic clone NOS16 was mapped with respect to BamHl, Hindlll, and Xhol. Human NOS15 was mapped with respect to BamHl. Sequencing of restriction fragments marked by asterisks establishes the relative positions of exon 5 1, exon 5 2, and exon 2 (E2) as indicated. 

The recycling function controls the output flow control function, which is the ability to control the enclosure output gateways that force out of the SSR the raw materials, raw parts, and raw part fragments marked as refuse by the recycling function. 

Valentine, J. L., Arnold, L. W. and Owens, M. (1994) Antiphencyclidine monoclonal Fab fragments markedly alter phencyclidine pharmacokinetics in rats. J. Pharmacol. Exp. Then 269, 1079-1085. 

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