Fragment isolation from agarose

Fig. 3 Appearance of fragments protected from micrococcal nuclease digestion following incubation of Xenopus sperm chromatin in homologous egg extract. Sperm nuclei were used without extract incubation (lane 2) or following incubation of 50 ng DNAZ/il egg LSS for 3 min (lane 3), 10 min (lane 4), 30 rain (lane 5), or 120 min (lane 6). Samples were diluted and the sperm nuclei were isolated by centrifugation. The samples were digested with micrococcal nuclease for 1 min and the DNA purified and separated by a 1.4% Tris-borate-EDTA agarose gel. Lane I shows molecular weight markers in lOO-base-pair increments.

Fig. 1. Twelve clones isolated from a rat brain cDNA library with labeled Pst/Pst fragment of v-erb-A were digested with Bgl I endonuclease and electro-phoresed on an agarose gel.

Figure 4. Suppression of DNA fragmentation caused by Trp-P-1 in P-NF-treated rat hepatocytes. Hepatocytes were isolated from P-naphthoflavone (P-NF) and corn oil (C)-treated rats and cultured for 20 h. These hepatocytes were treated with Trp-P-1 for 6 h, and DNA fragmentation was analyzed by 2% agarose gel electrophoresis.

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