Fractograms latex beads

Narrow particle fractions approaching a monodisperse distribution are particularly easy to treat and characterize when the above equations are applied to experimental data. Figure 2 shows an example of the elution profile (fractogram) obtained by running a mixture of four samples of "monodisperse" polystyrene latex beads. It is clear from the figure that a rather precise value of retention volume Vr can be identified with each bead size. With Vr known, it is easy to obtain R and X from Equation 5 and thence particle diameter d from Equation 4. This operation, as noted, yields diameters accurate to approximately 1-3%. 

Fig. 1 Fractograms of the polystyrene latex beads of 0.357 /xm (PSl) obtained by the direct injection of 1 julL of PSl (a) and by the concentration procedure of the PSl sample diluted in 10 mL of the carrier solution (b) using the conventional SdFFF technique, as well as of the a-Fe203 sample with nominal particle diameter of 0.271 tm diluted in 6 mL of the carrier solution obtained by the PBSdFFF concentration methodology (Ic).

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