Fraction collection oligonucleotides

High performance capillary electrophoresis was introduced originally as an analytical tool. Now that instruments are equipped with automated fraction collection, however, capillary electrophoresis can be used for micropreparative collection of individual peaks separated from a mixture. Using the fraction collection feature, nanomolar amounts of solute such as proteins, peptides, oligonucleotides can be collected in amounts sufficient for microsequencing. An intersample washing procedure and use of well-formed capillaries aid in the prevention of artifacts.44... [Pg.398]

Fig. 14. Fractionation of methylated oligonucleotides from free and bound rRNA. Bound ([ C]methyl) and free (pH]methyl) ribosomal RNA were mixed with cold carrier total RNA, digested with pancreatic RNase, and the resulting digest was separated on a 1 x 30 cm column of DEAF cellulose (Whatman DE32). The columns were eluted with an 800 ml gradient of 0-0.35 m NaCl, also containing 0.01 M Tris-Cl (pH 7.7) and 7 Murea. Fractions of 2.8 ml were collected every 5 min. No material was eluted beyond fraction 200. Top panel,...

$Fig. 14. Fractionation of methylated oligonucleotides from free and bound rRNA. Bound ([ C]methyl) and free (pH]methyl) ribosomal RNA were mixed with cold carrier total RNA, digested with pancreatic RNase, and the resulting digest was separated on a 1 x 30 cm column of DEAF cellulose (Whatman DE32). The columns were eluted with an 800 ml gradient of 0-0.35 m NaCl, also containing 0.01 M Tris-Cl (pH 7.7) and 7 Murea. Fractions of 2.8 ml were collected every 5 min. No material was eluted beyond fraction 200. Top panel,...$

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