Forward chemical genetics natural products

The synthesis of the natural-product-inspired indoloquinolizine compound collection, the HCS for mitotic inhibitors and the elucidation of the mode of action of centrocountin 1 is a demonstrative example of the forward-chemical genetics approach. It illustrates the workflow for the identification of biologically active small molecules in cells and target deconvolution. Centrocountin 1 was the most potent hit compound in a screen for mitotic inhibition and impairs the proper chromosome congression in cells. This results in chromosomal misalignment and... 

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