Methionyl-tRNA formyltransferase

METHIONYL-tRNA SYNTHETASE METHIONYL-tRNA FORMYLTRANSFERASE METHIONYL-tRNA SYNTHETASE AMINO-ACYL tRNA SYNTHETASES Method of continuous variation,... [Pg.761]

Schmitt, E., Blanquet, S., and Mechulam, Y. (1996). Structure of crystalline Escherichia colt methionyl-tRNA(f)Met formyltransferase comparison with glycinamide ribonucleotide formyltransferase. EMBO J., 15, 4749-4758. [Pg.75]

Formylation of methionylated tRNA " allows differentiation of the AUG start codon from internal AUG codons (14). MetRS aminoacylates tRNA" with methionine. A formyl group is linked covalently to the charged methionine via its amino moiety by the methionyl-tRNA formyltransferase enzyme, which uses N -formyl tetrahydrofolate as the formyl donor. This fMet-tRNA molecule binds directly to the P site of the ribosome to initiate protein synthesis, as compared with the A-site to which elongator tRNAs bind. [Pg.35]

Synthesis of fMet occurs on its tRNA. The tRNA is charged by MetRS with methionine, which then is formylated by methionyl-tRNA formyltransferase (MTF) in the presence of the formyl donor NlO-formyltetrahydrofolate (27). Initiation factor IF2 sequesters the fMet-tRNA and excludes it from the ribosomal A site. Instead, IF2-bound fMet-tRNA is transported to the ribosomal P site. EF-Tu GTP can bind methionyl-tRNA , although at a lower affinity than... [Pg.1893]

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