Formation of Bisindole Pyrrole

Two genes appear to be essential for indolocarbazole production in actinomycetes. One of the genes encodes a tryptophan oxidase (rebO, staO), while the second one codes for a heme-containing oxidase (rebD, staD). In their absence, no bisindole intermediates can be formed [23,33]. 

Studies made with recombinant strains of Strep, alhus showed that formation of the simplest bisindole intermediate during indolocarbazole biosynthesis required the coexpression of two genes rebO and rebD [33]. The said metabolite consisted of chromopyrrolic add (CPA, 37), which seemed to be an intermediate during 

The RebD enzyme was characterized as the first member of a new subfamily of heme-containing oxidases [34,36]. The enzyme acted as both a catalase and a CPA synthase, apparently converting two molecules of 7-chloroindole-3-pyruvic acid imine into ll,ll -dichloro-CPA. Formation of CPA by StaD, an enzyme homolog to RebD, was also confirmed for STA biosynthesis [35], 

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Formation of bisindole pyrrole rebO staO atO Amino oxidase... 

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