Folin and Denis’ reagent

Dissolve the residue in 0-5 ml of ethanol and add 0 5 ml of water followed by 0-4 ml of dilute hydrochloric acid, 0-8 ml of Folin and Denis reagent (see below) and 5 ml of water. Mix thoroughly, allow to stand for ten minutes and add 3 ml of 10 per cent sodium carbonate... 

A further colorimetric determination of stilboestrol depends on the reduction of Folin and Denis reagent by the phenolic groups of the stilboestrol molecule the method is applicable to tablets and the following details are modifications of those originally published by Tubis and Bloom. ... 

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