Fluorochrome labeled inhibitors

Bedner, E., Smolewski, P., Amstad, P. A., and Darzynkiewicz, Z. A. (2000). Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA) Correlation with DNA fragmentation. Exp. Cell Res. 259, 308—313. 

Fig. 6. Activation of caspases detected by the fluorochrome-labeled caspase (FLICA) inhibitors assay. FiL-60 cells were untreated (A), treated in culture with camptothecin to induce apoptosis (B) (ref. 26). The cells were then electrostatically attached to microscope slides, incubated with staining solution of FAM-VAD-FMK as described in the protocol, and their green fluorescence (integrated value and pixel of maximal intensity) measured by LSC. Note the appearance of apoptotic cell subpopulation characterized by the increased green fluorescence (above the marked threshold level of the maximal pixel) reflecting activation of caspases that bind FAM-VAD-FMK.

---