Fluorimetric analysis performance/limitations

In situ quantitation The fluorimetric analysis was performed in long-wavelength UV light (A c = 365 nm, Af, > 430 nm). The detection limit for thiamine was less than 3 ng per chromatogram zone. 

In situ quantitation The absorption-photometric determination in a reflectance mode was performed at A = 330 nm (detection limit ca. 40 ng per chromatogram zone). The fluorimetric analysis was carried out at =313 nm and An > 560 nm (detection limits ca. 10 ng per chromatogram zone) (Fig. 1). 

In situ quantitation The fluorimetric analysis of monoterpene glucosides could be performed with advantage at 2exc = 313 nm and 2 > 390 nm. The detection limits of arbutin and L-menthylglucoside were 1 — 5 ng and 15 ng substance per chromatogram respectively (Fig. 1). 

In situ quantitation The fluorimetric analysis was performed at A c = 405 mn and the fluorescent light was measured at Afi > 460 nm. The detection limits were... 

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