Fluorescers 1,3-diphenyl isobenzofuran

I>iphenylbenzo[c]thiophene, yellow needles, mp 118°C, is thermally stable. Its solution displays a green fluorescence. The compound can be prepared by ring transformation of 1,3-diphenyl-isobenzofuran with P4S10 in CS2. 

Attaching of fluorescent probes to PLP has also been used to determine PLP. Chauhan and Dakshinamurti employed reductive amination of PLP with methyl anthranilate and sodium cyanoborohydride to form a highly fluorescent amine (83). Durko et al. (54) prepared diphenyl-isobenzofurane derivatives of Be vitamers to enable fluorometric measurement of vitamin Be. However, the use of fluorescent probes are generally not suitable for routine analytical work, mainly because the unreacted fluorescent probe may interfere in Be vitamer quantification (83). 

Strong chemiluminescence was observed only in dimethylphthalate as solvent. Small quantities of water enhanced the light yield but oxygen had no distinct influence on the chemiluminescence [24 a]. Phthaloyl peroxide exhibits chemiluminescence with a series of other fluorescent compounds such as BPEA, pyrene, perylene, 1,3-diphenyl isobenzofuran, and fluorescein. 

Strong quenching effects were observed by 02,1,3-pentadiene, tri-t-butyl phenol and tetramethylethylene (in increasing order of effectiveness). As DP A and 1,3-diphenyl isobenzofuran act as fluorescers, but not 9,10-dibromoan-thracene, the active species appears to be in the singlet state, although quantitative results have not been reported. It was concluded from the kinetic and spectroscopic data that dioxetanedione as well as the 1,4-diradical are present not only in the... 

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