Room-temperature fluorescence description

Room temperature fluorescence induction was detected by a Dumont KM 2433 photomultiplier tube (S.20 surface) blocked with a Corning CS2-64 filter. Excitation of chloroplasts (5 yg chl/ml) in a mixture of 15 mM tricine-NaOH pH 7.8, 0.1 M sorbitol, 5 mM MgCl2, 10 mM NaCl 5 yM DCMU was accomplished by passing light from an illuminator (see lamp description above) through a Corning CS4-96 blue filter. The signal from the photomultiplier tube was stored on a Princeton Applied Research scan recorder (Model 4101) or recorded directly on a Houston 2000 X-Y recorder. Low temperature (77K) fluorescence emission spectra were obtained as described (Kyle, Zalik 1982). 

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