Fluorescence microscopy actin detection

In order to check if the toxin was able to exhibit activity toward cytoskeleton of neuroblastoma cells, Louzao et al. stained filamentous actin (F-actin) of the cells with Oregon Green 514 Phalloidin and detected the fluorescence with LSC and confocal microscopy. Neuroblastoma cells incubated with 30 jM gambierol for 4 h, did not show modifications in the amount of F-actin or its distribution, indicating a cellular integrity in treated cells (Figure 28.16). Same results were observed with CTX3. 

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