Fluorescence lifetime imaging spectroscopy FLIM

The first instrument combining time resolved fluorescence spectroscopy with microscopy, dates back to 1959 [2]. In this instrument, only single point measurements could be done, so strictly speaking no actual imaging was done. The first instrument measuring spatially resolved lifetimes was described in 1989 [3]. Various instruments were developed in the early 1990 s independently by different groups. Nowadays, there are many FLIM instruments, mostly used for biomedical apphcations. 

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