Fluorescence contour diagram

Intensity/wavelength/time cross-sectional diagrams (or time-resolved fluorescence "contour" diagrams) are generated using a weighted nonlinear least squares polynomial surface procedure (20). Area-normalized TRE spectra can be used for convenient pictorial representation, since the absolute emission intensity of individual time-resolved spectra vary substantially with time after excitation. 

Figure 3. Time-resolved fluorescence contour diagram for PACE in benzene at 25°C.

Van Duuren [37] examined the use of emission and excitation spectra in the identification of aromatic hyorocarbons. Contour diagrams of fluorescence activity at various excitation and emission wavelengths have been used as a means of identifying petroleum residues. 

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