Fluorescence Assays Using Membrane Potential Dyes

Fluorescence Assays Using Membrane Potential Dyes... [Pg.13]

Several functional sodium channel assays that use membrane potential sensing dyes and pharmacological activators have been described [99,106, 108,112]. In addition, there are recent reports of fluorescent sodium channel assays using changes in an electric field applied across cells to activate sodium channels [110, 111], thus avoiding potential interactions of the test compound with the channel activator. [Pg.139]

Note 3 The membrane potential sensitive fluorescence dye-based assays do not reflect the behavior of the ion channels and are prone to fluorescence quenching. The assays can be used in early phase of drug discovery for lead identification. [Pg.56]