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Flow microfluorometry

Flow microfluorometry (fluorescence activated cell sorting, FACS)... [Pg.207]

To give some idea of the results CHO cells pulse labelled with [3H]thymidine were stained with either ethidium bromide or using the acriflavin-Feulgen method (again staining DNA) and submitted to flow microfluorometry and cell sorting. [Pg.208]

Fig. 10.13. Distribution of cells separated by flow microfluorometry. CHO cells were pulse labelled for 15 min with [3H]thymidine (1 / Ci/ml) and stained with ethidium bromide. They were then submitted to flow microfluorometry and cell sorting on the basis of cellular DNA content. Cells from the indicated portions (sort 1, 2 and 3) were then subjected to autoradiography and shown to contain respectively 4%, 93% and 19% of the cells labelled. (Reproduced from Kraemer et al., 1973, with kind permission of the authors and publisher.)... Fig. 10.13. Distribution of cells separated by flow microfluorometry. CHO cells were pulse labelled for 15 min with [3H]thymidine (1 / Ci/ml) and stained with ethidium bromide. They were then submitted to flow microfluorometry and cell sorting on the basis of cellular DNA content. Cells from the indicated portions (sort 1, 2 and 3) were then subjected to autoradiography and shown to contain respectively 4%, 93% and 19% of the cells labelled. (Reproduced from Kraemer et al., 1973, with kind permission of the authors and publisher.)...
Coulson, P. B. Bishop, A. O. Lenarduzzi, R. Quantitation of cellular deoxyribonucleic acid by flow microfluorometry. J. Histochem. Cytochem. 1977,25,1147-1153. [Pg.6]

Capron A. Cytophilic IgE on human blood and tissue eosinophils detection by flow microfluorometry. J Immunol 1985 134 3013-3018. [Pg.81]


See other pages where Flow microfluorometry is mentioned: [Pg.233]    [Pg.239]    [Pg.233]    [Pg.239]   
See also in sourсe #XX -- [ Pg.207 ]




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