Flavoprotein oxidase assay

Potato Phenol Oxidase. The studies of Kubowitz on the copper oxidase of potatoes were part of the efforts of Warburg s institute to find enzyme systems that could catalyze oxygen consumption coupled with substrate oxidation. The enzyme was purified on the basis of an assay involving transfer of electrons from pyridine nucleotides to o-quinone and from the resulting catechol to oxygen (I). The reduction of catalytic quantities of quinone was probably catalyzed by a flavoprotein present in the Zwischenferment preparation used to reduce TPN. In the presence of... 

---