Flavonoid neohesperidosides

Webby, R.F. and Boase, M.R., Peonidin 3-0-neohesperidoside and other flavonoids from Cyclamen persicum petals. Phytochemistry, 52, 939, 1999. 

Dauguet, J.C. et al., 8-Methoxykaempferol 3-neohesperidoside and other flavonoids from bee pollen of Crataegus monogyna, Phytochemistry, 33, 1503, 1993. 

Both directly and indirectly, flavonoid composition affects the quality of individual citrus cultivars and hybrids. Some cultivars, such as the sour orange, are directly affected by the presence of bitter neohesperidosides to the point they are unpalatable. The quality of other cultivars may be indirectly affected due to the presence of bitterness suppressing flavone neohesperidosides. Certain hybrids, such as the K early, also contain bitter flavanone glycosides at levels which reduce its acceptability. Therefore, the knowledge of the relative type and... 

One of the major challenges inthe structural analysis of flavonoids using mass spectrometry is the difficulty of differentiating between isomers. Rutinosides and neohesperidosides are the two most common flavonoid diglycosides. These isomers differ only by the... 

Because the flavonoid glycosides are non-volatile, GC has not routinely been used for flavonoid analysis. However, Greaser et al. (1992) have developed a method to analyze the hydrolyzed flavanone content by GC-MS. The method involves solvent extraction of the tissues, hydroylsis of the flavonoids to their respective aglycones, trimethylsilation, and analysis by GC-MS. While the method will accurately identify and quantify the flavonoid aglycones present in the sample, it cannot determine the relative amounts of the neohesperidosides and rutinosides, and would therefore only be able to give an estimation of the amount of bitter flavonoids in a sample. 

Jourdan PS, Mansell RL, Weiler EW (1982) Radioimmunoassay for the citrus bitter principle, naringin, and related flavonoid-7-0-(3-neohesperidosides. J Med Plant Res 44 82-86... 

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