Flavanones sample preparation

HPLC is the method of choice for the separation of complex mixtures containing non-volatile compounds such as various flavonoids in extracts prepared from different samples. A survey of literatures revealed that most researchers have used Cjg-reversed stationary phases, which proved to be superior to the normal phase technique. The reversed phases are suitable for separating flavonoids in a wide range of polarities, as Vande Casteele et al. have demonstrated the separation of 141 flavonoids from polar triglycosides to relatively non-polar polymetoxy-lated aglycones belonging to the classes of flavones, flavonols, flavanones, dihydroflavonols, chalcones, and dihydrochalcones. 

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