Flavanone glycosides, HPLC

Table 5 Isocratic HPLC Analysis for Flavanone Glycosides HPLC condition...

For the HPLC of flavanone glycosides in citrus, isocratic elution is often preferred because of the simplicity of the major flavanone glycosides occurring in citrus, and the flavanone glycosides are also present in citrus juice at fairly high levels. Furthermore, isocratic separations require no re-equilibration time between analyses and therefore are sometimes faster when only a few components are to be analyzed. 

Table 7 Gradient HPLC for the Simultaneous Analysis of Flavanone Glycosides and Polymethoxylated Flavones...

Fig. 12 HPLC separation of flavanone glycosides in grapefruit juice and orange juice.

Fig. 13 HPLC separation of flavanone glycosides and polymethoxylated flavones (PMFs) in orange juice spiked with didymin and PMFs.

Cautela, D. Laratta, B. Santelli, F. Trifiro, A. Servillo, L. Castaldo, D. 2008. Estimating Bergamot juice adulteration of lemon juice by high-performance liquid chromatography (HPLC) analysis of flavanone glycosides. J. Agric. Food Chem. 56 5407-5414. 

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