Flank PNDMA

One application of 1 % PNDMA in olive oil produces no visible change on the flank or on the nipple under both macroscopic and histological examination after 3, 6 or 14 hours. 

Fig. 16. Guinea pig sensitized to PNDMA. Flank 14 hours after a single application of 1 % PNDMA in olive oil acanthosis, no epidermal lesions...

Two weeks after this second sensitization, we tested one flank with 0.1% DNCB in acetone and the other with 1% PNDMA in olive oil. The 2 patch tests were strongly positive (N. Hunziker and coll., 1964). Thus, we can say that previous sensitization with a strong eczematogen, such as DNCB, has not prevented further sensitization with a weaker eczematogen, as Landsteiner, Rostenberg and Sulzberger (1939) have also stated. 

In guinea pigs sensitized with CA, PA and PNDMA, spongiosis are less frequently found. They are present almost always on the nipple, but rarely on the flank. 

---