FLAG tag

Purification of FLAG-Tagged Eukaryotic Initiation Factor 2B Complexes, Subcomplexes, and Fragments from Saccharomyces cerevisiae... 

Figure 1.2 Schematic outlining the purification protocol. The principal steps of purifying FLAG-tagged eIF2B proteins from yeast whole cell extracts are outlined in the diagram for details, refer to the main text.

Immunoaffinity purification of elF3 containing FLAG-tagged... 

MHCK B, recombinant from Dictyostelium discoideum cells as FLAG-tagged protein [15]) [15]... 

GST, calmodulin-binding peptide, His-, FLAG-tag, protein A, glycoprotein D of HSV), or to enable its detection and/or selection, i.e. tags based on protein-reporters (/1-galactosidase, GFP, CAT, hGH) (Makrides, 1999). Tags can be fused to N- or C-terminal ends of the protein and a site for proteolytic cleavage is commonly included to eliminate the tag upon exploitation of its functionality. The proteases most commonly used are thrombin, enterokinase, factor Xa, and TEV (catalytic domain of Nia, the nuclear inclusion protein from tobacco etch virus). 

FLAG-tag—polypeptide protein tag that is added to a recombinant expressed protein... 

Both naturally processed FasL and Flag-tagged, recombinant soluble FasL bound to recombinant Fas and to the surface of Fas positive cells. Surprisingly, however, these soluble FasLs were almost completely unable to induce... 

Figure 4. Expression of caspase-resistant mut-PARP-1 induces a persistent poly(ADP-ribosyl)ation of ONAS1L3 during etoposide-induced apoptosis in osteosarcoma cells. Osteosatcoma cells expressing either DNAS1L3 or both mut-PARP-1 and DNAS1L3 were incubated for the indicated times with 70 pM etoposide, after which the recombinant His6-FLAG-tagged proteins were precipitated and then subjected to immunoblot analysis with antibodies to poly(ADP-ribose) polymer (PAiy. Reprinted with permission from Boulares et al, J Biol Chem 277(l) 372-378, 2002 The American Society for Biochemistry and Molecular Biology, Inc.

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