Fit strategy

Obviously the results become increasingly complex with the number of intermediates being increased. A fit of an experimental curve seems to be hardly useful for more complicated models unless most of the fit parameters can be directly estimated from the experimental curve. This is, for example, possible, if all peaks are well separated. 

An effective fit strategy starts with the extrapolation of the heat capacities of the pure native and denatured states 

Additionally, using the relation AG°(Ti/2) = AH Tii2) — Ti/2AS (Ti/2), it is seen that the transition temperature Tj/2 increases with increasing protein concentration according to 

When the T1/2 values for the two transitions axe exchanged a simple one-step reaction is observed (dash-dotted curve). The low stability of the monomers would favour early denaturation, however, the stability of the oligomerized native state (dimer) dominates the overall transition. If such a peak were decomposed into two transitions using a fitting routine, the transition temperatures and Tj 2 well as the transition enthalpies Ai/j and would be obtained with large uncertainties. 

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Organisms exhibit tremendous plasticity in their abifity to modulate behavior, depending on the perceived availability of resources in the environment. This determination may occur through analysis of bottom-up data such as caloric intake or top-down data such as ambient temperature. Integration of these various sources of data then allows for the development of fitness strategies tailored to... 

To optimise the model parameters, the fitting strategy involves the simultaneous treatment of all isothermal and non-isothermal cure experiments with and/or without mobility restrictions of a thermosetting system of afixed initial composition. The temperature range is largely extended to obtain an accurate estimate for all fitting parameters of the cure rate model. 

This study, based on the size-fitting strategy, showed that a slight change of ionic diameters of lanthanide cations greatly affected the diastereoselectivity as well as enantioselectivity of the reaction. 

Fitting experimental data with the above model is not straightforward and requires both an extanded set of data and the appropriate fitting strategy, as demonstrated below with shear viscosity data on a series of Ti02 filled polystyrene at 180°C, as published by Minagawa and White [/. Appl. Polym. Sci, 20,501-523,1976]. 

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