FIP units of lipase

Using optimized procedures, up to about 11,000 FIP units of lipase can be... 

To about 10% of the normal pancreatic secretion, 100,000 FIP units of lipase are required per meal [80]. This means that in order to facilitate patient compliance, foe galenic formulation should contain the maximum amount of panoreatin possible per dose. Earlier studies suggest a minimum of 30,000 FIP units per meal [81], but in recent years higher amounts per meal have been recommended [80,82]. 

Using optimized procedures, up to about 11,000 FIP units of lipase can be extracted from 1 g of pancreas gland. Some pancreatin preparations may contain relatively important amounts of purines, which in patients receiving large doses of drugs can lead to hyperuricosuria and hyperuricemia [75]. 

Pancreatin is described in the different pharmacopeia as a slightly brown, amorphous powder with a faint, characteristic odor. It is slowly and incompletely soluble in water it is not recommended to filter the solution as the lipolytic enzymes and part of the proteolytic activity may be retained on the filter. Most pharmacopeia stipulate the minimum units of amylase (12,000 FIP units/g), lipase (15,000 FIP units/g), and protease (1000 FIP units/g) activity, but give no specifications on the content of the other components such as phospholipase A2, carboxylester lipase, colipase, and individual proteolytic enzymes. It... 

Calculation One unit of enzyme activity (FIP Unit) is defined as that quantity of a standard lipase preparation (Fungi Lipase-International FIP Standard) that liberates the equivalent of 1 (imol of fatty acid per minute from the Substrate Emulsion under the described assay conditions. The specific activity is expressed in international FIP units per milligram of the Sample Preparation. 

Reference Standard Solution Dissolve an aliquot of Fungi Lipase-International FIP Standard (International Commission on Pharmaceutical Enzymes F.I.P., Center for Standards of the Federation Internationale Pharmaceutique, Harelbekes-traat 72, B-9000 Gent, Belgium) in a 1% sodium chloride solution and dilute it to obtain a solution of 2.4 to 3.6 FIP microbial lipase units per milliliter. Prepare this solution fresh. 

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