Filter paper extracellular

The strains were cultured on Mandels medium + 1% citrus pectin for 5 days and the enzymatic activities of culture filtrates were determined on three substrates citrus pectin, polygalacturonic acid and filter paper, (a) extracellular proteins are in p.g/ml. (b) p>ectinolytic activities on pectin (PC) and on polygalacturonic acid (TO) and Pectin esterase (PE) are in units/ml. (c) total cellulolytic activity (filter paper, fp) are in mg of liberated reducing sugars/ml. 

Figure 1. Cellulose degradation, cell growth and extracellular filter-paper activity in a culture of Thermoactinomyces sp., strain YX. 40 L batch fermentor, 55°C, pH 7.2 (5).

The two genes encoding ECB deacylase subunits from A. utahensis were cloned and overexpressed by S. lividans as soluble extracellular proteins [25], The recombinant S. lividans was grown in a 10-liter fermenter under conditions described previously [30], After 2 days of growth, the culture was filtered through 1 Whatman filter paper. The recombinant ECB deacylase from the culture filtrate... 

Figure 2. Zone electrophoretic patterns of (a) extracellular cellulose from 0.5% filter-paper culture and (b) cell-bound cellulose from 0.5% cellobiose culture of Ps. fluorescens...

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