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Filter diagonalization technique

The technique of two dimensional electrophoresis with two identical procedures applied in a right angle to each other, does not offer additional resolution and would be therefore useless unless there is some reaction that alters the charge of some members in the mixture of separated components performed between individual runs. Then the reacted members would deviate from the diagonal where all the other non-reacted components would be localized. An example of this procedure is that of MikeS and HolySovsky [19] and Hartley et al. [20]. Using this procedure disulphide peptides from a tryptic hydrolysate of an enzyme were analyzed. The hydrolysate is separated in the first run on a filter paper strip. The separated peptides are then oxidized with performic acid vapour by means of which the S-S bridges are broken... [Pg.418]

See other pages where Filter diagonalization technique is mentioned: [Pg.591]    [Pg.699]    [Pg.699]    [Pg.591]    [Pg.699]    [Pg.699]    [Pg.2316]    [Pg.552]    [Pg.660]    [Pg.330]    [Pg.192]    [Pg.2316]    [Pg.660]    [Pg.3135]    [Pg.141]    [Pg.276]    [Pg.125]    [Pg.325]    [Pg.327]    [Pg.152]    [Pg.465]    [Pg.450]    [Pg.264]    [Pg.169]    [Pg.130]    [Pg.338]    [Pg.271]    [Pg.149]    [Pg.1071]   
See also in sourсe #XX -- [ Pg.5 , Pg.3135 ]



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