Film development agitation types

Separate the sandwich after 8 min. Place the gel into 25 ml of gel fixing solution and prepare for staining with Coomassie blue or with silver. Place the film into a dry petri dish (for best contrast on a light box) and slowly pour 30 ml of developing buffer into the dish. Do not agitate the dish. Temperature and pH of the buffer depend on type and amount of the protease investigated and should reflect optimal reaction conditions. 

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