Fibroblast growth factor inhibitors

M Botta, F Manetti, F Corelli. Fibroblast growth factors and their inhibitors. Curr Pharm Design 6 1897-1924, 2000. 

Wilson DK, Nakano T, Petrash JM, Quiocho FA. 1.7 A structure of FR-1, a fibroblast growth factor-induced member of the aldo-keto reductase family complexed with coenzyme and inhibitor. Biochemistry 1995 34 14323-14330. 

Murzin AG, Lesk AM, Chothia C. b- Trefoil Fold patterns of structure and sequence in the kunitz inhibitors interleukins-1J3 and la and fibroblast growth factors. J Mol Biol 1992 223 531-543. 

Jellinek, D., Green, L., Bell, C., Lynott, C.K., Gill, N., Vargeese, C. et al. (1995) Potent 2 -amino-deoxypyrimidine RNA inhibitors of basic fibroblast growth factor. Biochemistry, 34, 11363-11372. 

Heparin has been used in enzyme purification such as recombinant human mast cell tryptase. The purified enzyme is fully active [12]. Heparin-based affinity chromatography also permitted the isolation of growth factors such as basic fibroblast growth factor (bFGF). The affinity is lower when bFGF is complexed with acidic gelatin [13]. The elution of synthetic TFPI (tissue factor pathway inhibitor) peptidic fragments on immobilized heparin has allowed one to find the peptidic sequence responsible for the TFPI-heparin interaction [14]. 

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