Fibrinogen radii

The very small blank hole at the center of the fibrinogen disc, increasing in size with dilution of plasma and fibrinogen solution used, had a radius A2 corresponding to a liquid layer height of H2 = R — V(R2 —A2) (see Figure 3 and values in Tables I and II). The significance of H2 is discussed later. 

Some experiments were carried out with normal intact plasma, but in addition to antiserum to fibrinogen, antiserum to kininogens (kindly provided by R. W. Colman, Temple University School of Medicine, Philadelphia, PA) was applied. A lens with an approximately 147-mm radius of... 

More detailed studies (using lenses of about 147mm radius of curvature) indicated that a set of concentric rings of proteins is left by plasma under these conditions. Diluted to about. 02 to. 01% it will leave albumin centrally, surrounded by immunoglobulins (IgG) at about 1%, it will leave a central disk of IgG surrounded by fibronectin and fibrinogen, while at about 20%, it will leave a solid disc of fibringoen with a central hole containing IgG surrounded by a fine line of fibronectin (11). 

Solving, we find that a equals 67 nm and b equals 2.2 nm. If fibrinogen were a sphere, its radius would be about 16 nm. 

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