Fibrin unstabilized

Table 1 demonstrates that high lytic activity is displayed by the complexes of thrombin and fibrinogen as well as by that of heparin and plasmin. These complexes, as was shown in Ref.35), affect the unstabilized fibrin only and supress simultaneously the activity of the fibrin-stabilizing factor. Qualitatively similar results were observed in in vivo experiments. 

Fig. 8. Kinetics of unstabilized fibrin hydrolysis with complexes of immobilized heparin with fibrinogen (1), thrombin (2), fibrinolysin (plasmin) (3), and serum albumin (4),06)...

The kinetics of the lytic effect displayed by the complexes of immobilized heparin with thrombin and fibrinogen, in distinction from those with plasmin, are described by their saturation curves. The observed slowing down of the dissolution of unstabilized fibrin is probably due to the inhibiton of the lytic activity of the complexes by the soluble products of the reaction. In fact, as it was shown in Ref. 106, further addition of immobilized heparin-protein complex to partially hydrolyzed fibrin results in a complete recovery of the dissolution rate. 

Factor XIII deficiency. There is no expected effect on any of the clotting time measurements. The susceptibility to plasmin suggests an unstabilized clot. The solubility in urea is diagnostic for un-cross-linked fibrin. 

Heparin-serotonin complexation to form an entity with anticoagulant activity and lytic activity different from that of plasmin with respect to the fact that it lysed unstabilized fibrin clots in the presence of EACA has been reported.93 Evidence of a protective action of vitamin C in deep vein thrombosis has also been reported.94... 

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