Fermentor, microbial batch

Bioreactors that use enzymes but not microbial cells could be regarded as fermentors in the broadest sense. Although their modes of operation are similar to those of microbial fermentors, fed-batch operation is seldom practiced for enzyme reactors. The basic equations for batch and continuous reactors for... 

Intermediates in the biosynthetic route are 3-dehydroshikimic acid, protocate-chuic acid and catechol (Scheme 7.16). Optimization of microbial ds,ds-muconic acid synthesis required the expression of three enzymes not typically found in Escherichia coli [42cj. E. coli WNl/pWN2.248 was developed that synthesized 36.8 g of ds,ds-muconic acid in a 22% (mol/mol) yield from glucose after 48 h of culturing under fed-batch fermentor conditions. Optimization of the carbon flow directed into ds,ds-muconic acid biosynthesis and manipulation of enzyme activities were aimed at avoiding accumulation of biosynthetic intermediates. 

Microbial Reduction of 4-Benzyloxy-3-Methanesulfonyl-amino-2 -Bromoacetophenone. The microbial reduction of 4-benzyloxy-3-methanesulfonylamino-2 -bromoacetophe-none (20) (Fig. 5B) to the corresponding (/ )-alcohol (21) has been demonstrated (32) using Sphingomonas paucimo-bilis SC 16113. The growth of S. paucimobilis SC 16113 was carried out in a 750-L fermentor cells (60 kg) harvested from the fermentor were used to conduct the biotransformation in 10- and 200-L preparative batches. The cells were... 

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