Fermentation of Ergoline Derivatives

Preferably the incubation of the cultures is effected, generally, at a pH from 6.5 to 4.0, a time from 3 to 18 days, a temperature from 15° to 28° C and aerobic conditions. Further details will appear from the following examples. The first three examples illustrate obtaining an extract directly from the culture medium. 

A part of the spores of an inoculum grown as described in Example 1 and stored for 9 months at 4.° C was suspended and equal amounts thereof were placed into round bottom flasks of 500 mL. contents. Each flask received 120 mL. of a culture medium containing 10% saccharose, 1.5% ammonium citrate, 0.1% Ca(N03)2, 0.025% KH2P04, 0.01% KCl, 0.03% MgS04, 0.001% FeS04, 0.0004% ZnS04 distilled water pH 5.5 sterilization 30 min at 0.5 at. above atmospheric. 

The flasks were then subjected to shaking under the same condition as before. 

Parallel to this test there were determined the contents of alkaloids of the mycelium which had been separated by suction filtration and of the filtrate. It was found that the ergometrine was completely contained in the filtrate and that in addition 90% of the total ergotoxines was in the filtrate. 

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