Factorial Design for the Preparation of Affinity Resins

The large number of variables to be optimized necessitates the application of a mathematical method. The literature describes the preparation of a vast number of affinity resins. The resin should by well-suited for the intended application. 

But how good is good Should it be possible to improve the purification by modifying the immobilization conditions (salt type and concentration, ligand density or pH)  

these problems are solved by trial and error, changing one at a time. However, this is not efficient with such a large number of variables. 

In Table 3, the interaction between pH and ligand concentration is also considered. A low or high pH value may decrease the concentration of a peptide or protein ligand by hydrolysis. 

The signs of Y, can be taken from Table 3. An arbitrary binding capacity value can be calculated from the summary equation  

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