Fab region

IgE binds to tissue receptor sites through its F region, leaving the Fab region free to react with the allergen. The half-life of IgE is 2 days... 

Messenger RNA is isolated from the tissue source selected to contain the Ab-secreting cells. Spleen, bone marrow, tonsil, and lymph node have all been successfully used as a source Copy DNA (cDNA) is then generated by reverse transcription and Fv or Fab regions amplified by PCR. 

Fig. 1.1 The immunoglobulin molecule each immunoglobulin molecule is composed of two identical heavy (CH + VH) and two identical light chains (CL + VL). The antigen binds to the Fab region, which varies according to the specificity of the antibody. The rest of the domains (blue) are constant. The classes of the antibody molecules differ based on the Fc region of the heavy chain see Color Insert)...

The Fab region of the mAb reacts with its antigen on the cell surface. 

Cytotoxicity by the Fab region of the IgG on the target cell surface, resulting in the... 

Noncompetitive Immunoassays. In a typical noncompetitive assay for an antigen, a capture antibody is first passively adsorbed or covalently bound to the surface of a solid phase. Various sequences in which the capture antibody can be attached are shown in Box 9-2. The simplest involves direct attachment to the solid phase. However, this can lead to some loss of antibody binding capacity because of steric factors or attachment of the antibody via its Fab region. To protect the binding properties of the antibody, more complex sequences have been devised. For example, the solid support can be coated with an antispecies antibody, and then... 

Immunocytochemistry uses antibodies against IgGs. Antibodies or IgG molecules are generated to other IgG molecules by injecting purified IgG molecules from one species into another species. In the case of mouse IgG injected into rabbit, it will produce rabbit anti-mouse IgG antibodies. Antibodies made against an IgG will only bind to the constant region or Fab region of the IgG. 

Variable region - the portion of an antibody with the unique configuration that specifically binds the epitope with the fraction antigen binding (Fab) region. 

Polystyrene beads are known for their capacity to bind immunoglobin G globulins nonspecifically, by hydrophobic interaction, so that the immu-nospecificity of the Fab region is preserved. Because of the dipolar nature of the phenyl ring, polystyrene probably represents a surface capable of extraordinary interaction with hydrophobic regions of proteins. 

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